Journal: Drug Metabolism and Disposition

Article Title: Impact of the transcription factor nuclear factor 1 B T>C polymorphism on clozapine metabolism in vivo and expression of intestinal transporters in vitro

doi: 10.1016/j.dmd.2025.100100

Figure Lengend Snippet: Effect of NFIB rs28379954 T>C diplotype on metabolic ratios in smoking patients adjusting for age, sex, and sampling time. The data are analyzed using linear mixed model. The figure displays the fold change (95% CIs) in NFIB CT carriers (heterozygous for the target allele) versus NFIB TT carriers (homozygous for the target allele). Significant effect was observed for the metabolic ratio of N -desmethylclozapine cysteine: DCL-CYS/DCL (fold change: 1.89; CI: 1.04–3.44; P = .0384). contains the effect estimates, fold changes, and 95% CIs of the rest of the metabolites. CI, confidence interval; DCL, N -desmethylclozapine; DCL-CYS/DCL, DCL-cysteinyl; NFIB, nuclear factor 1 B.

Article Snippet: A predesigned TaqMan-based real-time polymerase chain reaction assay NFIB rs28379954 T>C (Thermo Fisher Scientific) was used for the determination of NFIB genotype.

Techniques: Sampling

Journal: Drug Metabolism and Disposition

Article Title: Impact of the transcription factor nuclear factor 1 B T>C polymorphism on clozapine metabolism in vivo and expression of intestinal transporters in vitro

doi: 10.1016/j.dmd.2025.100100

Figure Lengend Snippet: Effect of NFIB rs28379954 T>C diplotype on metabolic ratios in nonsmoking patients adjusting for age, sex, and sampling time. The data are analyzed using linear mixed model. The figure displays the fold change (95% CIs) in NFIB CT carriers (heterozygous for the target allele) versus NFIB TT carriers (homozygous for the target allele). No significant effect was observed for the metabolic ratios of the 30 metabolites. contains the effect estimates, fold changes, and 95% CIs of all the metabolites. CI, confidence interval; NFIB, nuclear factor 1 B.

Article Snippet: A predesigned TaqMan-based real-time polymerase chain reaction assay NFIB rs28379954 T>C (Thermo Fisher Scientific) was used for the determination of NFIB genotype.

Techniques: Sampling

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Primer sequences used for reverse transcription polymerase chain reaction analysis of NELIN.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Primer sequences used for reverse transcription polymerase chain reaction analysis of SM22α.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Reverse transcription polymerase chain reaction analysis revealed weak mRNA expression of NELIN in the vascular smooth muscle cells (VSMCs) of the experimental group, but strong expression in the control group. (A) Expected length of the PCR products was 611 bp (NELIN) and 312 bp (β-actin). Lanes: N, control group; X, DNA marker DL 2000; and P, experimental group. (B) Downregulation of NELIN mRNA expression in the VSMCs from the varicose vein tissue. Data are presented as the mean ± standard deviation.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Reverse transcription polymerase chain reaction analysis revealed weak mRNA expression of SM22α in the vascular smooth muscle cells (VSMCs) of the experimental group, but strong expression in the control group. (A) Expected length of the PCR products was 232 bp (SM22α) and 472 bp (GADPH). Lanes: N, control group; X, DNA marker DL 2000; and P, experimental group. (B) Downregulation of SM22α mRNA expression in the VSMCs from the varicose vein tissue. Data are presented as the mean ± standard deviation.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Standard Deviation